Glassy-winged sharpshooter biocontrol

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in French Polynesia

Monitoring of the parasitoïd efficiency

- Parasitoïd dispersion

 

Several methods of detection were used to monitor the dispersion of the parasitoid after it was released and established. GWSS eggs were collected and stored in Petri dishes, and parasitism rates were determined. Yellow sticky cards and pan traps were placed to detect adult parasitoids (G. ashmeadi is attracted to yellow). These methods permit quick and accurate monitoring of parasitoïd spread and enables determination of dispersion rates after release.

GWSS eggs
pan trap yellow sticky cards

 

- Parasitism rate

GWSS egg masses are collected from regularly monitored field sites to calculate parasitism rates. Parasitized GWSS eggs are darker because of the developing parasitoid than uparastized healthy GWSS eggs. Parasitized GWSS eggs from which G. ashmeadi successfully emerged show characteristic circular emergence holes made by the adult as it chews through the GWSS eggshell and leaf to escape.

GWSS eggs fresh vs hatched and healthy vs parasitized

 

- GWSS abundance

 

A map of GWSS population densities is made for each infested islands. GWSS populations are monitored by sweep netting plants for a fixed time period and counting the numbers of GWSS nymphs and adults that are collected. At the same time, GWSS numbers are monitored with yellow sticky cards and time count from regularly monitored field sites.

sweep netting
time count yellow sticky cards
regularly monitored field sites in Tahiti
Jérôme N. Petit © 2006 - Gump station